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Record #Source (e.g. developer/organization)Acronym (e.g. ISO, IEC)Identifier (e.g. document designation number)Title of documentScope/descriptionKeywordsType of DocumentIssue area(s) this applies toPrimary category that this applies toIntended stakeholdersLastModif
127Nanotechnology Characterization LaboratoryNCLGTA-5LLC-PK1 Kidney Cell Apoptosis AssayThis protocol describes the monitoring of nanoparticle treated porcine renal proximal tubule...LLC-PK1e. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
126Nanotechnology Characterization LaboratoryNCLGTA-2 Hep G2 Hepatocarcinoma Cytotoxicity AssayThis protocol describes the cytotoxicity testing of nanoparticle formulations in human...Hep g2, MTT, LDHe. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
125LLC-PK1 Kidney Cytotoxicity AssayGTA-1LLC-PK1 Kidney Cytotoxicity Assay This protocol describes the cytotoxicity testing of nanoparticle formulations in porcine proximal...LDH, MTTe. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
124Nanotechnology Characterization LaboratoryNCLGTA-7Hepatocyte Primary ROS AssayThis protocol describes the testing of nanoparticle formulations for reactive oxygen species(ROS)...hepatocytes, SDe. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
123Nanotechnology Characterization LaboratoryNCLGTA-4Hep G2 Hepatocyte Li pid Peroxidation Assay This protocol describes the analysis of human hepatocarcinoma cells (Hep G2) for lipid...Hep G2, MDAe. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
122Nanotechnology Characterization LaboratoryNCLGTA-3Hep G2 Hepatocyte Glutathione AssayThis protocol describes the analysis of human hepatocarcinoma cells (Hep G2) for reduced and...glutathione, HepG2,e. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
121Nanotechnology Characterization LaboratoryNCLITA-17In Vitro Induction of Leukoc yte Procoagulant Activity by Nanoparticles Leukocyte procoagulant activity (PCA) is accepted as an important component in the onset of...PCA, DIC, APLe. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
120Nanotechnology Characterization LaboratoryNCLITA-14 Analysis of Nanoparticle Effect s on Maturation of Monocyte Derived Dendritic Cells In Vitro Dendritic cells (DC) are antigen-presenting cells. They play an important role in initiation and...dendritic cells, cytotoxicitye. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
119Nanotechnology Characterization LaboratoryNCL ITA-11Measurement of Nanoparticle Effect s on Cytotoxic Activity of NK Cells by Label-Free RT-CES System NK cells carry small granules in their cytoplasm which contain special proteins such as perforin...perforin, NK, granzymese. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Energy e.g. Energy storage, energy efficiency2014-07-16
118Nanotechnology Characterization LaboratoryNCLITA-9Phagocytosis AssayThis document describes a protocol for evaluation of nanoparticle internalization by phagocytic...phagolysosomee. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
117Nanotechnology Characterization LaboratoryNCLITA-8Chemotaxis AssayThis document provides a protocol for rapid, quantitative measure of the chemoattractant capacity...chemokines, chemoattractant capacity, prostaglandins, cytokinese. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
116National Institute of Standards and Technology-Nanotechnology Characterization LaboratoryNCLITA-7Detection of Nitric Oxide Producti on by the Macrophage Cell Line RAW264.7 This document describes a protocol for quantitative determination of nitrite (NO2-) a stable...cytotoxicity, nitricoxide, NO 2, NO 3e. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
115Nanotechnology Characterization LaboratoryNCLITA-6Leukocyte Proliferation AssayThis document describes a protocol for assessing the effect of a nanoparticle formulation on the...PHA-M,lymphocytese. SpecificationHealth, Safety, and EnvironmentChemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
114Nanotechnology Characterization LaboratoryNCLITA-3Mouse Granulocyte-Macrophage Colony-Forming Unit Assay Myelosuppression is a common dose limiting toxicity of cytotoxic oncology drugs. Nanoparticles...BM, granulocyte macrophagecolony, Myelosuppressione. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
113Nanotechnology Characterization LaboratoryNCLITA-12Coagulation AssaysThis document describes a protocol for assessing the effect a nanoparticle formulation may have...APTT, TT, Coagulatione. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
112Nanotechnology Characterization LaboratoryNCL ITA-5.2Quantitative Analysis of Complement Activation This document describes a protocol for quantitative determination of complement activation by an...EIAe. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
111Nanotechnology Characterization LaboratoryNCLITA-5.1Qualitative Analysis of Tota l Complement Activation by Western Blot This document describes a protocol for qualitative determination of total complement activation...Western blot, lectine. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-16
110Nanotechnology Characterization LaboratoryNCLITA-4NCL Method ITA-4 Analysis of Nanoparticle Interaction with Plasma Proteins by 2D PAGE Proteins bind the surfaces of nanoparticles, and biological materials in general, immediately...nanoparticles, protein binding, phagocytic cells e. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-10
109Nanotechnology Characterization LaboratoryNCLITA-2Analysis of Platelet AggregationThis document describes a procedure for analysis of platelet aggregation (1-6). Platelets are...thrombocyte, megakaryocyte, Plateletse. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-10
108Nanotechnology Characterization LaboratoryNCLITA-1 Analysis of Hemolytic Properties of Nanoparticles The hemolytic potential of all intravenously administered pharmaceuticals must be evaluated....in vivo, hemolytic potential, nanoparticles2014-07-10
107Nanotechnology Characterization LaboratoryNCLITA-1 Analysis of Hemolytic Properties of Nanoparticles The hemolytic potential of all intravenously administered pharmaceuticals must be evaluated....in vivo, hemolytic potential, nanoparticles2014-07-10
106Nanotechnology Characterization LaboratoryNCLGTA-13This protocol assumes an intermediate level of scientific competency with regard to techniques, instrumentation, and safety procedures. Rudimentary assay details have been omitted for the sake of brevity. NCL Method GTA - 13Characterization of drug release under physiological conditions is very important for prediction...hydrophilic nanoparticle platform, RBC, in vivoe. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-10
105Nanotechnology Characterization LaboratoryNCLCell BindingCell Binding/Internalization NCL has methods to asses targe ting by measuring the inhibition coefficient (Ki) and binding...Cell Bindinge. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-10
104Nanotechnology Characterization LaboratoryNCLSTE-3Detection of MycoplasmaNanoparticles submitted to the NCL may be subjected to testing for mycoplasma when deemed...hybridoma, mycoplasmae. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-10
103Nanotechnology Characterization LaboratoryNCLSTE-2Detection of Microbial ContaminationThe intended purpose of this assay is to avoid introduction of microbial contamination into in...in vitro, in vivo, nanomateriale. SpecificationMeasurement and Characterizations
Health, Safety, and Environment
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals2014-07-10
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