124

Selected Document Category:	Published Document
Source:	Nanotechnology Characterization Laboratory
Anticipated Publication Year:
Publication Year:	2010
Acronym:	NCL
Identifier:	GTA-7
Document Title:	Hepatocyte Primary ROS Assay
url:	http://ncl.cancer.gov/NCL_Method_GTA-7.pdf
Scope:	This protocol describes the testing of nanoparticle formulations for reactive oxygen species(ROS) generation in male Sprague-Dawley (SD) primary hepatocytes, as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes a fluorescent redox active probe. Primary hepatocytes were chosen since they have a greater metabolic activity than hepatocyte cell lines.
Keywords:	hepatocytes, SD
Type of Document:	e. Specification
Issues Areas:	Measurement and Characterizations Health, Safety, and Environment
Primary Category:	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:	A
Submitter's e-mail:	[email protected]
Submitter's Company:	ANSI
Additional Comments:
Contact for Additional Info:	http://ncl.cancer.gov/working_assay-cascade_download.asp

Selected Document Category:	Published Document
Source:	Nanotechnology Characterization Laboratory
Anticipated Publication Year:
Publication Year:	2010
Acronym:	NCL
Identifier:	GTA-7
Document Title:	Hepatocyte Primary ROS Assay
url:	http://ncl.cancer.gov/NCL_Method_GTA-7.pdf
Scope:	This protocol describes the testing of nanoparticle formulations for reactive oxygen species(ROS) generation in male Sprague-Dawley (SD) primary hepatocytes, as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes a fluorescent redox active probe. Primary hepatocytes were chosen since they have a greater metabolic activity than hepatocyte cell lines.
Keywords:	hepatocytes, SD
Type of Document:	e. Specification
Issues Areas:	Measurement and Characterizations Health, Safety, and Environment
Primary Category:	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:	A
Submitter's e-mail:	[email protected]
Submitter's Company:	ANSI
Additional Comments:
Contact for Additional Info:	http://ncl.cancer.gov/working_assay-cascade_download.asp

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