|Selected Document Category:||Published Document|
|Anticipated Publication Year:|
|Document Title:||Standard Test Method for Evaluation of the Effect of Nanoparticulate Materials on the Formation of Mouse Granulocyte-Macrophage Colonies|
Significance and Use
Stem cells of hematopoietic origin are pluripotential and may be particularly sensitive to the effects of stimulation by nanoparticulate materials.
The effect of particles on macrophage responses has an extensive history and can be assessed by Practice F 1903. The test method described here will assess the effect on stem cells which can be progenitor cells to the macrophage line.
This test method provides a protocol for quantitative analysis of the effect of nanoparticulate materials in physiologic solution on granulocyte-macrophage colony-forming units.
This test method employs murine bone marrow hematopoietic stem cells which proliferate and differentiate to form discrete cell clusters or colonies which are counted.
This test method is part of the in vitro preclinical characterization cascade for nanoparticulate materials for systemic administration in medical applications.
|Keywords:||bone marrow; CFU-GM; clonogenic assay; granulocyte; macrophage; myelosuppression; stem cells|
|Type of Document:||a. Standard|
|Issues Areas:||Measurement and Characterizations|
|Goal or Need:|
|Is it Being Implemented:|
|Submitter's Name:||Kate McClung|
|Submitter's Company:||ASTM International|
|Contact for Additional Info:|