Loading...
 

Tracker Item History

View Tracker Item

Version Date User Field ID Field Difference
4 194 Identifier (e.g. document designation number)
-ISO/AWI 19007 +19007
      195 Title of document
-Nanotechnologies - In vitro MTS Assay for measuring the cytotoxic effect of nanoparticles +Nanotechnologies - In Vitro MTS Assay For Measuring The Cytotoxic Effect Of Nanoparticles
      196 Scope/description
-This document specifies guidance on evaluation of the in vitro toxicity response from cells exposed to nanoparticles using the MTS Assay or equivalent cytotoxicity test. In this test, cellular viabillty is determined by measuring the extent of reduction of {3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium} (MTS) to formazan by mitochondrial reductases in viable cells.This standard is intended to be used in biological testing laboratories that are competent in the culture and growth of cells and the evaluation of in vitro toxicity of chemicals.The cell line used in this assay is mouse murine myeloid (RAW 264.7). This document is applicable to materials that consist of nano-objects such as nano-particles, nano-powders, nano-fibres, nano-tubes, nano-wires, as well as aggregates and agglomerates of these materials. +ISO 19007:2018 specifies a method for evaluating the effects of nano-objects and their aggregates and agglomerates (NOAA) on cellular viability using the MTS assay. The assay design includes performance requirements and control experiments to identify and manage variability in the assay results.
 +ISO 19007:2018 is applicable to the use of a 96-well plate.
      198 Publication year
- +2018
      201 Primary category that this applies to
-Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals +
      213 URL Link
- +https://webstore.ansi.org/Standards/ISO/ISO190072018
      214 Anticipated publication year
-2015 +
      215 Select Document Category
-Document Awaiting to be Published +Published Document
      223 Contact for additional comments and inquiries
- +Heather Benko, [email protected]
2 2014-05-13 10:29 hbenko 195 Title of document
-Nanotechnologies -- Modified 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) Assay for measuring the effect of nanoparticles on cell viability +Nanotechnologies - In vitro MTS Assay for measuring the cytotoxic effect of nanoparticles
::::