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Selected Document Category:Published Document
Source:National Institute of Standards and Technology-Nanotechnology Characterization Laboratory
Anticipated Publication Year:
Publication Year:2011
Acronym:NCL
Identifier:ITA-7
Document Title:Detection of Nitric Oxide Producti on by the Macrophage Cell Line RAW264.7
url:http://ncl.cancer.gov/NCL_Method_ITA-7.pdf
Scope:This document describes a protocol for quantitative determination of nitrite (NO2-) a stable oxidative end-product of the antimicrobial effector molecule nitricoxide in cell culture medium(1, 2). The protocol is used to evaluate the capability of nanomaterials to induce nitric oxide production by macrophages. Nitric oxide secreted by macrophages has a half-life of seconds; it interacts with a number of different molecular targets, resulting in cytotoxicity. In the presence of oxygen and water, nitric oxide interacts with it self to generate other reactive nitrogen oxide intermediates and ultimately decomposes to form nitrite (NO 2) and nitrate (NO 3).
Keywords:cytotoxicity, nitricoxide, NO 2, NO 3
Type of Document:e. Specification
Issues Areas:Measurement and Characterizations
Health, Safety, and Environment
Primary Category:Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:Admin
Submitter's e-mail:[email protected]
Submitter's Company:ANSI
Additional Comments:
Contact for Additional Info:http://ncl.cancer.gov/working_assay-cascade_download.asp



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