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Selected Document Category:	Published Document
Source:	Nanotechnology Characterization Laboratory
Anticipated Publication Year:
Publication Year:	2011
Acronym:	NCL
Identifier:	ITA-14
Document Title:	Analysis of Nanoparticle Effect s on Maturation of Monocyte Derived Dendritic Cells In Vitro
url:	http://ncl.cancer.gov/NCL_Method_ITA-14.pdf
Scope:	Dendritic cells (DC) are antigen-presenting cells. They play an important role in initiation and regulation of the cellular immune response. Maturation of dendritic cells is achieved by inflammatory cytokines (e.g. TNF), or pathogen associated molecular patterns (e.g. bacterial lipopolysaccharide, LPS). This protocol can be used to study the in vitro efficacy of nanoparticle-based vaccine formulations. In addition, it can also be usedto assess the potential cytotoxicity of nanoparticle formulations to dendritic cells, and the nano particle effects on DC maturation in response to traditional inflammatory stimuli such as endotoxin.
Keywords:	dendritic cells, cytotoxicity
Type of Document:	e. Specification
Issues Areas:	Measurement and Characterizations Health, Safety, and Environment
Primary Category:	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:	Admin
Submitter's e-mail:	[email protected]
Submitter's Company:	ANSI
Additional Comments:
Contact for Additional Info:	http://ncl.cancer.gov/working_assay-cascade_download.asp

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