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Selected Document Category:	Published Document
Source:	Nanotechnology Characterization Laboratory
Anticipated Publication Year:
Publication Year:	2010
Acronym:	NCL
Identifier:	GTA-3
Document Title:	Hep G2 Hepatocyte Glutathione Assay
url:	http://ncl.cancer.gov/NCL_Method_GTA-3.pdf
Scope:	This protocol describes the analysis of human hepatocarcinoma cells (Hep G2) for reduced and oxidized glutathione, following treatment with nanoparticle formulations, as part of the NCL in vitro preclinical characterization cascade. A shift from reduced to oxidized glutathione isindicative of oxidative stress, while a decrease in the overall reduced glutathione pool is indicative of conjugative metabolism or impaired synthesis (1). This protocol utilizes a colorimetric method for glutathione determination.
Keywords:	glutathione, HepG2,
Type of Document:	e. Specification
Issues Areas:	Measurement and Characterizations Health, Safety, and Environment
Primary Category:	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:	Admin
Submitter's e-mail:	[email protected]
Submitter's Company:	ANSI
Additional Comments:
Contact for Additional Info:	http://ncl.cancer.gov/working_assay-cascade_download.asp

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