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Selected Document Category:Published Document
Source:LLC-PK1 Kidney Cytotoxicity Assay
Anticipated Publication Year:
Publication Year:2010
Acronym:
Identifier:GTA-1
Document Title:LLC-PK1 Kidney Cytotoxicity Assay
url:http://ncl.cancer.gov/NCL_Method_GTA-1.pdf
Scope:

This protocol describes the cytotoxicity testing of nanoparticle formulations in porcine proximal tubule cells (LLC-PK1), as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes two methods
for estimation of cytotoxicity, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT)reduction and lactate dehydrogenase (LDH) release(1, 2).

Keywords:LDH, MTT
Type of Document:e. Specification
Issues Areas:Measurement and Characterizations
Health, Safety, and Environment
Primary Category:Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:Admin
Submitter's e-mail:[email protected]
Submitter's Company:ANSI
Additional Comments:
Contact for Additional Info:http://ncl.cancer.gov/working_assay-cascade_download.asp
Selected Document Category:Published Document
Source:LLC-PK1 Kidney Cytotoxicity Assay
Anticipated Publication Year:
Publication Year:2010
Acronym:
Identifier:GTA-1
Document Title:LLC-PK1 Kidney Cytotoxicity Assay
url:http://ncl.cancer.gov/NCL_Method_GTA-1.pdf
Scope:

This protocol describes the cytotoxicity testing of nanoparticle formulations in porcine proximal tubule cells (LLC-PK1), as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes two methods
for estimation of cytotoxicity, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT)reduction and lactate dehydrogenase (LDH) release(1, 2).

Keywords:LDH, MTT
Type of Document:e. Specification
Issues Areas:Measurement and Characterizations
Health, Safety, and Environment
Primary Category:Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:Admin
Submitter's e-mail:[email protected]
Submitter's Company:ANSI
Additional Comments:
Contact for Additional Info:http://ncl.cancer.gov/working_assay-cascade_download.asp



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