128

Selected Document Category:	Published Document
Source:	Nanotechnology Characterization Laboratory
Anticipated Publication Year:
Publication Year:	2010
Acronym:	NCL
Identifier:	GTA-6
Document Title:	HepG2 Hepatocarcinoma Apoptosis Assay
url:	http://ncl.cancer.gov/NCL_Method_GTA-6.pdf
Scope:	This protocol describes the monitoring of nanoparticle treated human hepatocarcinoma cells (HepG2) for apoptosis, as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes a fluorescent method to determine the degree of caspase-3 activation.
Keywords:	HepG2, caspase-3 activation
Type of Document:	e. Specification
Issues Areas:	Measurement and Characterizations
Primary Category:	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:	Admin
Submitter's e-mail:	[email protected]
Submitter's Company:	ANSI
Additional Comments:
Contact for Additional Info:	http://ncl.cancer.gov/working_assay-cascade_download.asp

::::