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Selected Document Category:Published Document
Source:Nanotechnology Characterization Laboratory
Anticipated Publication Year:
Publication Year:2011
Acronym:NCL
Identifier:GTA-14
Document Title:Hep G2 Hepatocarcinoma Homogeneous Apoptosis Assay
url:http://ncl.cancer.gov/NCL_Method_GTA-14.pdf
Scope:This protocol describes the monitoring of nanoparticle treated human hepatocarcinoma cells (HepG2) for apoptosis, as part of the in vitro NCL preclinical characterization cascade (1, 2). The protocol utilizes a fluorescent method to measure Caspase-3/7 activation. This is a homogeneous assay that does not require cell isolation, as required for HepG2 Hepatocarcinoma Apoptosis Assay (NCL Method GTA-6).
Keywords:Hepg2, Caspase-3/7 activation
Type of Document:e. Specification
Issues Areas:Measurement and Characterizations
Health, Safety, and Environment
Primary Category:Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:Admin
Submitter's e-mail:[email protected]
Submitter's Company:ANSI
Additional Comments:
Contact for Additional Info:http://ncl.cancer.gov/working_assay-cascade_download.asp
Selected Document Category:Published Document
Source:Nanotechnology Characterization Laboratory
Anticipated Publication Year:
Publication Year:2011
Acronym:NCL
Identifier:GTA-14
Document Title:Hep G2 Hepatocarcinoma Homogeneous Apoptosis Assay
url:http://ncl.cancer.gov/NCL_Method_GTA-14.pdf
Scope:This protocol describes the monitoring of nanoparticle treated human hepatocarcinoma cells (HepG2) for apoptosis, as part of the in vitro NCL preclinical characterization cascade (1, 2). The protocol utilizes a fluorescent method to measure Caspase-3/7 activation. This is a homogeneous assay that does not require cell isolation, as required for HepG2 Hepatocarcinoma Apoptosis Assay (NCL Method GTA-6).
Keywords:Hepg2, Caspase-3/7 activation
Type of Document:e. Specification
Issues Areas:Measurement and Characterizations
Health, Safety, and Environment
Primary Category:Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:Admin
Submitter's e-mail:[email protected]
Submitter's Company:ANSI
Additional Comments:
Contact for Additional Info:http://ncl.cancer.gov/working_assay-cascade_download.asp



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