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Selected Document Category:	Published Document
Source:	Nanotechnology Characterization Laboratory
Anticipated Publication Year:
Publication Year:	2013
Acronym:	NCL
Identifier:	GTA-11
Document Title:	Autophagic Dysfunction Assay: Qualitative Analysis of MAP LC3 - I to LC3 - II C onversion by Western Blot
url:	http://ncl.cancer.gov/NCL_Method_GTA-11.pdf
Scope:	Lysosomal dysfunction is recognized as a potential toxic mechanism for xenobiotics that can result in various pathological states (1). There is concern that nanopaticles, in particular, may cause lysosomal pathologies, since they are likely to accumulate within lysosomes (2). Lysosomal dysfunction could potentially result from nanoparticle biopersistance, or inhibition of lysosomal enzymes, such as inhibition of phospholipase resulting in phospholipidosis, or inhibition of lysosomal protein degradation resulting in lysosomal overload
Keywords:	xenobiotics, lysosomal pathologies, phospholipase
Type of Document:	e. Specification
Issues Areas:	Measurement and Characterizations Health, Safety, and Environment
Primary Category:	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:	Admin
Submitter's e-mail:	[email protected]
Submitter's Company:	ANSI
Additional Comments:
Contact for Additional Info:	http://ncl.cancer.gov/working_assay-cascade_download.asp

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