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Selected Document Category:Published Document
Source:International Organization for Standardization
Anticipated Publication Year:2022
Publication Year:
Identifier:TS 4988
Document Title:Nanotechnologies — Bioavailability assessment of manufactured nanomaterials in an aquatic environment using Tetrahymena sp

This technical specification provides a reliable and repeatable method for simultaneous assessment of both exposure and toxicity of MNOs using Tetrahymena sp. The ingested, internalized material (MNOs) indicates aquatic exposure.

This document is intended to be used by all the centers working with nano(eco)toxicity of MNOs and capable of culturing of Tetrahymena sp. The method uses Tetrahymena sp. to assess exposure and effects of MNOs. In addition, the test could be used by centers (laboratories) interested in investigating the biological interaction of MNOs with living cells.

This method is applicable to nano-objects such as nanoparticles, nanofibers of certain size (in a μm size range), nanoplates, as well as their aggregates and agglomerates.

Keywords:ecotoxicity, nanoparticles, nanofibers
Type of Document:a. Standard
Issues Areas:Health, Safety, and Environment
Primary Category:
Additional Categories
Goal or Need:Tetrahymena phagocytic activity is a cost-effective physiological endpoint, which can act as a very early and sensitive indicator for the toxic effect of various xenobiotic compounds as well as an indication of internalization / bioassimilation of xenobiotics. This endpoint could also serve as a measure of exposure to MNOs in any suspension of MNOs where their cellular internalization is of interest.
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:Heather Benko
Submitter's e-mail:[email protected]
Submitter's Company:ANSI
Additional Comments:
Contact for Additional Info:Heather Benko ([email protected])