Selected Document Category:Published Document
Source:ASTM International
Anticipated Publication Year:
Publication Year:2008
Document Title:Standard Test Method for Evaluation of the Effect of Nanoparticulate Materials on the Formation of Mouse Granulocyte-Macrophage Colonies

Significance and Use

Stem cells of hematopoietic origin are pluripotential and may be particularly sensitive to the effects of stimulation by nanoparticulate materials.

The effect of particles on macrophage responses has an extensive history and can be assessed by Practice F 1903. The test method described here will assess the effect on stem cells which can be progenitor cells to the macrophage line.


This test method provides a protocol for quantitative analysis of the effect of nanoparticulate materials in physiologic solution on granulocyte-macrophage colony-forming units.

This test method employs murine bone marrow hematopoietic stem cells which proliferate and differentiate to form discrete cell clusters or colonies which are counted.

This test method is part of the in vitro preclinical characterization cascade for nanoparticulate materials for systemic administration in medical applications.

Keywords:bone marrow; CFU-GM; clonogenic assay; granulocyte; macrophage; myelosuppression; stem cells
Type of Document:a. Standard
Issues Areas:Measurement and Characterizations
Primary Category:
Additional CategoriesMedical
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:Kate McClung
Submitter's e-mail:kmcclung@astm.org
Submitter's Company:ASTM International
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