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Selected Document Category:	Published Document
Source:	Nanotechnology Characterization Laboratory
Anticipated Publication Year:
Publication Year:	2011
Acronym:	NCL
Identifier:	GTA-14
Document Title:	Hep G2 Hepatocarcinoma Homogeneous Apoptosis Assay
url:	http://ncl.cancer.gov/NCL_Method_GTA-14.pdf
Scope:	This protocol describes the monitoring of nanoparticle treated human hepatocarcinoma cells (HepG2) for apoptosis, as part of the in vitro NCL preclinical characterization cascade (1, 2). The protocol utilizes a fluorescent method to measure Caspase-3/7 activation. This is a homogeneous assay that does not require cell isolation, as required for HepG2 Hepatocarcinoma Apoptosis Assay (NCL Method GTA-6).
Keywords:	Hepg2, Caspase-3/7 activation
Type of Document:	e. Specification
Issues Areas:	Measurement and Characterizations Health, Safety, and Environment
Primary Category:	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:	Admin
Submitter's e-mail:	[email protected]
Submitter's Company:	ANSI
Additional Comments:
Contact for Additional Info:	http://ncl.cancer.gov/working_assay-cascade_download.asp

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