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Selected Document Category:Published Document
Source:Nanotechnology Characterization Laboratory
Anticipated Publication Year:
Publication Year:2010
Acronym:NCL
Identifier:GTA-2
Document Title:Hep G2 Hepatocarcinoma Cytotoxicity Assay
url:http://ncl.cancer.gov/NCL_Method_GTA-2.pdf
Scope:

This protocol describes the cytotoxicity testing of nanoparticle formulations in human hepatocarcinoma cells (Hep G2), as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes two methods
for estimation of cytotoxicity, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release(1, 2).

Keywords:Hep g2, MTT, LDH
Type of Document:e. Specification
Issues Areas:Measurement and Characterizations
Health, Safety, and Environment
Primary Category:Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
Additional Categories
Goal or Need:
Intended Stakeholders:
Is it Being Implemented:
Submitter's Name:Admin
Submitter's e-mail:[email protected]
Submitter's Company:ANSI
Additional Comments:
Contact for Additional Info:http://ncl.cancer.gov/working_assay-cascade_download.asp



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