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122
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Published Document
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2010
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Nanotechnology Characterization Laboratory
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NCL
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GTA-3
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Hep G2 Hepatocyte Glutathione Assay
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http://ncl.cancer.gov/NCL_Method_GTA-3.pdf
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This protocol describes the analysis of human hepatocarcinoma cells (Hep G2) for reduced and...
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glutathione, HepG2,
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
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Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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Admin
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[email protected]
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ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
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EF
|
sculley
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Editors
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2014-07-16 |
121
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Published Document
|
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2011
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Nanotechnology Characterization Laboratory
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NCL
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ITA-17
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In Vitro Induction of Leukoc yte Procoagulant Activity by Nanoparticles
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http://ncl.cancer.gov/NCL_Method_ITA-17.pdf
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Leukocyte procoagulant activity (PCA) is accepted as an important component in the onset of...
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PCA, DIC, APL
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
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Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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Admin
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[email protected]
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ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
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EF
|
sculley
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Editors
|
2014-07-16 |
120
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Published Document
|
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2011
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Nanotechnology Characterization Laboratory
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NCL
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ITA-14
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Analysis of Nanoparticle Effect s on Maturation of Monocyte Derived Dendritic Cells In Vitro
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http://ncl.cancer.gov/NCL_Method_ITA-14.pdf
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Dendritic cells (DC) are antigen-presenting cells. They play an important role in initiation and...
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dendritic cells, cytotoxicity
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
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Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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Admin
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[email protected]
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ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
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EF
|
sculley
|
Editors
|
2014-07-16 |
119
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Published Document
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2009
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Nanotechnology Characterization Laboratory
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NCL
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ITA-11
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Measurement of Nanoparticle Effect s on Cytotoxic Activity of NK Cells by Label-Free RT-CES System
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http://ncl.cancer.gov/NCL_Method_ITA-11.pdf
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NK cells carry small granules in their cytoplasm which contain special proteins such as perforin...
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perforin, NK, granzymes
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
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Energy e.g. Energy storage, energy efficiency
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Admin
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[email protected]
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ANSI
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http://ncl.cancer.gov/working_assay-cascade_dow...
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EF
|
sculley
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Editors
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2014-07-16 |
118
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Published Document
|
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2011
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Nanotechnology Characterization Laboratory
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NCL
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ITA-9
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Phagocytosis Assay
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http://ncl.cancer.gov/NCL_Method_ITA-9.pdf
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This document describes a protocol for evaluation of nanoparticle internalization by phagocytic...
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phagolysosome
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
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Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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Admin
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[email protected]
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ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-16 |
117
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Published Document
|
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2011
|
Nanotechnology Characterization Laboratory
|
NCL
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ITA-8
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Chemotaxis Assay
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http://ncl.cancer.gov/NCL_Method_ITA-8.pdf
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This document provides a protocol for rapid, quantitative measure of the chemoattractant capacity...
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chemokines, chemoattractant capacity, prostaglandins, cytokines
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
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Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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Admin
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[email protected]
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ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
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EF
|
sculley
|
Editors
|
2014-07-16 |
116
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Published Document
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2011
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National Institute of Standards and Technology-Nanotechnology Characterization Laboratory
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NCL
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ITA-7
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Detection of Nitric Oxide Producti on by the Macrophage Cell Line RAW264.7
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http://ncl.cancer.gov/NCL_Method_ITA-7.pdf
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This document describes a protocol for quantitative determination of nitrite (NO2-) a stable...
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cytotoxicity, nitricoxide, NO 2, NO 3
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e. Specification
|
Measurement and Characterizations
Health, Safety, and Environment
|
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
|
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Admin
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[email protected]
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ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
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EF
|
sculley
|
Editors
|
2014-07-16 |
115
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Published Document
|
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2011
|
Nanotechnology Characterization Laboratory
|
NCL
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ITA-6
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Leukocyte Proliferation Assay
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http://ncl.cancer.gov/NCL_Method_ITA-6.pdf
|
This document describes a protocol for assessing the effect of a nanoparticle formulation on the...
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PHA-M,lymphocytes
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e. Specification
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Health, Safety, and Environment
|
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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Admin
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[email protected]
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ANSI
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http://ncl.cancer.gov/working_assay-cascade_dow...
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EF
|
sculley
|
Editors
|
2014-07-16 |
114
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Published Document
|
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2009
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Nanotechnology Characterization Laboratory
|
NCL
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ITA-3
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Mouse Granulocyte-Macrophage Colony-Forming Unit Assay
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http://ncl.cancer.gov/NCL_Method_ITA-3.pdf
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Myelosuppression is a common dose limiting toxicity of cytotoxic oncology drugs. Nanoparticles...
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BM, granulocyte macrophagecolony, Myelosuppression
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
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Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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Admin
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[email protected]
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ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
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EF
|
sculley
|
Editors
|
2014-07-16 |
113
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Published Document
|
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2011
|
Nanotechnology Characterization Laboratory
|
NCL
|
ITA-12
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Coagulation Assays
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http://ncl.cancer.gov/NCL_Method_ITA-12.pdf
|
This document describes a protocol for assessing the effect a nanoparticle formulation may have...
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APTT, TT, Coagulation
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
|
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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Admin
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[email protected]
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ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-16 |
112
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Published Document
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2010
|
Nanotechnology Characterization Laboratory
|
NCL
|
ITA-5.2
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Quantitative Analysis of Complement Activation
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http://ncl.cancer.gov/NCL_Method_ITA-5.2.pdf
|
This document describes a protocol for quantitative determination of complement activation by an...
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EIA
|
e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
|
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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Admin
|
[email protected]
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ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-16 |
111
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Published Document
|
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2010
|
Nanotechnology Characterization Laboratory
|
NCL
|
ITA-5.1
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Qualitative Analysis of Tota l Complement Activation by Western Blot
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http://ncl.cancer.gov/NCL_Method_ITA-5.1.pdf
|
This document describes a protocol for qualitative determination of total complement
activation...
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Western blot, lectin
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
|
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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Admin
|
[email protected]
|
ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-16 |
110
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Published Document
|
|
2010
|
Nanotechnology Characterization Laboratory
|
NCL
|
ITA-4
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NCL Method ITA-4 Analysis of Nanoparticle Interaction with Plasma Proteins by 2D PAGE
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http://ncl.cancer.gov/NCL_Method_ITA-4.pdf
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Proteins bind the surfaces of nanoparticles, and biological materials in general, immediately...
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nanoparticles, protein binding, phagocytic cells
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
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Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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Admin
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[email protected]
|
ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-10 |
109
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Published Document
|
|
2009
|
Nanotechnology Characterization Laboratory
|
NCL
|
ITA-2
|
Analysis of Platelet Aggregation
|
http://ncl.cancer.gov/NCL_Method_ITA-2.pdf
|
This document describes a procedure for analysis of platelet aggregation
(1-6). Platelets are...
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thrombocyte, megakaryocyte, Platelets
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
|
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
|
|
|
|
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Admin
|
[email protected]
|
ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-10 |
108
|
Published Document
|
|
2009
|
Nanotechnology Characterization Laboratory
|
NCL
|
ITA-1
|
Analysis of Hemolytic Properties of Nanoparticles
|
http://ncl.cancer.gov/NCL_Method_ITA-1.pdf
|
The hemolytic potential of all intravenously administered pharmaceuticals must be evaluated....
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in vivo, hemolytic potential, nanoparticles
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Admin
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[email protected]
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ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-10 |
107
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Published Document
|
|
2009
|
Nanotechnology Characterization Laboratory
|
NCL
|
ITA-1
|
Analysis of Hemolytic Properties of Nanoparticles
|
http://ncl.cancer.gov/NCL_Method_ITA-1.pdf
|
The hemolytic potential of all intravenously administered pharmaceuticals must be evaluated....
|
in vivo, hemolytic potential, nanoparticles
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Admin
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[email protected]
|
ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-10 |
106
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Published Document
|
|
2013
|
Nanotechnology Characterization Laboratory
|
NCL
|
GTA-13
|
This protocol assumes an intermediate level of scientific competency with regard to techniques, instrumentation, and safety procedures. Rudimentary assay details have been omitted for the sake of brevity. NCL Method GTA - 13
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http://ncl.cancer.gov/NCL_Method_GTA-13.pdf
|
Characterization of drug release under physiological conditions is very important for prediction...
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hydrophilic nanoparticle platform, RBC, in vivo
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
|
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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|
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Admin
|
[email protected]
|
ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-10 |
105
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Published Document
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|
2010
|
Nanotechnology Characterization Laboratory
|
NCL
|
Cell Binding
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Cell Binding/Internalization
|
http://ncl.cancer.gov/NCL_Method_Cell_Binding.pdf
|
NCL has methods to asses targe
ting by measuring the inhibition
coefficient (Ki) and binding...
|
Cell Binding
|
e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
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Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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Admin
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[email protected]
|
ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-10 |
104
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Published Document
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2011
|
Nanotechnology Characterization Laboratory
|
NCL
|
STE-3
|
Detection of Mycoplasma
|
http://ncl.cancer.gov/NCL_Method_STE-3.pdf
|
Nanoparticles submitted to the NCL may be subjected to testing for mycoplasma when
deemed...
|
hybridoma, mycoplasma
|
e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
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Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
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|
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Admin
|
[email protected]
|
ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-10 |
103
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Published Document
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2011
|
Nanotechnology Characterization Laboratory
|
NCL
|
STE-2
|
Detection of Microbial Contamination
|
http://ncl.cancer.gov/NCL_Method_STE-2.pdf
|
The intended purpose of this assay is to avoid introduction of microbial contamination into in...
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in vitro, in vivo, nanomaterial
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e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
|
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
|
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A
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[email protected]
|
ANSI
|
|
http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-10 |
102
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Published Document
|
|
2011
|
Nanotechnology Characterization Laboratory
|
NCL
|
STE - 1.3
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Detection and Quantification of Gr am Negative Bacterial Endotoxin Contamination in Nanopart icle Formulations by Gel-Clot LAL Assay
|
http://ncl.cancer.gov/NCL_Method_STE-1.3.pdf
|
This document discusses the quantitative detection of Gram negative bacterial endotoxin in...
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BacterialEndotoxin, LAL
|
e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
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Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
|
|
|
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Admin
|
[email protected]
|
ANSI
|
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http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-10 |
101
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Published Document
|
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2011
|
Nanotechnology Characterization Laboratory
|
NCL
|
STE - 1.2
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Detection and Quantification of Gr am Negative Bacterial Endotoxin Contamination in Nanopart icle Formulations by Kinetic Turbidity LAL Assay
|
http://ncl.cancer.gov/NCL_Method_STE-1.2.pdf
|
This document describes a protocol for a quantitative detection of Gram negative bacterial...
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Bacterial Endotoxin, LAL
|
e. Specification
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Measurement and Characterizations
Health, Safety, and Environment
|
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
|
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Admin
|
[email protected]
|
ANSI
|
|
http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-10 |
100
|
Published Document
|
|
2011
|
Nanotechnology Characterization Laboratory
|
NCL
|
STE-1.1
|
Detection and Quantification of Gr am Negative Bacterial Endotoxin Contamination in Nanopart icle Formulations by End Point Chromogenic LAL Assay
|
http://ncl.cancer.gov/NCL_Method_STE-1.1.pdf
|
This document describes a protocol for a quantitative detection of Gram negative bacterial...
|
endotoxin,LAL, QCL-1000
|
e. Specification
|
Measurement and Characterizations
Health, Safety, and Environment
|
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
|
|
|
|
|
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Admin
|
[email protected]
|
ANSI
|
|
http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-10 |
97
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Published Document
|
|
2009
|
National Institute of Standards and Technology-Nanotechnology Characterization Laboratory
|
NIST-NCL
|
PCC-2
|
Measuring Zeta Potential of Nanoparticles
|
http://ncl.cancer.gov/NCL_Method_PCC-2.pdf
|
This protocol describes the measurement of zeta potential for nanomaterial samples in aqueous...
|
Zeta potential, nanomaterial
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e. Specification
|
Measurement and Characterizations
Health, Safety, and Environment
|
Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals
|
|
|
|
|
|
Admin
|
[email protected]
|
ANSI
|
|
http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-09 |
99
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Published Document
|
|
2010
|
National Institute of Standards and Technology-Nanotechnology Characterization Laboratory
|
NIST-NCL
|
PCC-13
|
Measuring the pH of Na noparticle Suspensions
|
http://ncl.cancer.gov/NCL_Method_PCC-13.pdf
|
This document provides a protocol for measuring the pH of dilute nanoparticle suspensions of...
|
NIST RM, nanoparticlesuspensions
|
e. Specification
|
Measurement and Characterizations
Health, Safety, and Environment
|
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|
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Admin
|
[email protected]
|
ANSI
|
|
http://ncl.cancer.gov/working_assay-cascade_dow...
|
EF
|
sculley
|
Editors
|
2014-07-08 |