Data Form

Items 226

Record #	Select Document Category	Publication year	Source (e.g. developer/organization)	Acronym (e.g. ISO, IEC)	Identifier (e.g. document designation number)	Title of document	URL Link	Scope/description	Keywords	Type of Document	Issue area(s) this applies to	Primary category that this applies to	Submitter's name	Email address	Submitter's company/organization	Contact for additional comments and inquiries	(This field is for ANSI use only)	login	group	Last modified
127	Published Document	2010	Nanotechnology Characterization Laboratory	NCL	GTA-5	LLC-PK1 Kidney Cell Apoptosis Assay	http://ncl.cancer.gov/NCL_Method_GTA-5.pdf	This protocol describes the monitoring of nanoparticle treated porcine renal proximal tubule...	LLC-PK1	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
126	Published Document	2010	Nanotechnology Characterization Laboratory	NCL	GTA-2	Hep G2 Hepatocarcinoma Cytotoxicity Assay	http://ncl.cancer.gov/NCL_Method_GTA-2.pdf	This protocol describes the cytotoxicity testing of nanoparticle formulations in human...	Hep g2, MTT, LDH	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
125	Published Document	2010	LLC-PK1 Kidney Cytotoxicity Assay		GTA-1	LLC-PK1 Kidney Cytotoxicity Assay	http://ncl.cancer.gov/NCL_Method_GTA-1.pdf	This protocol describes the cytotoxicity testing of nanoparticle formulations in porcine proximal...	LDH, MTT	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
124	Published Document	2010	Nanotechnology Characterization Laboratory	NCL	GTA-7	Hepatocyte Primary ROS Assay	http://ncl.cancer.gov/NCL_Method_GTA-7.pdf	This protocol describes the testing of nanoparticle formulations for reactive oxygen species(ROS)...	hepatocytes, SD	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	A	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
123	Published Document	2010	Nanotechnology Characterization Laboratory	NCL	GTA-4	Hep G2 Hepatocyte Li pid Peroxidation Assay	http://ncl.cancer.gov/NCL_Method_GTA-4.pdf	This protocol describes the analysis of human hepatocarcinoma cells (Hep G2) for lipid...	Hep G2, MDA	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
122	Published Document	2010	Nanotechnology Characterization Laboratory	NCL	GTA-3	Hep G2 Hepatocyte Glutathione Assay	http://ncl.cancer.gov/NCL_Method_GTA-3.pdf	This protocol describes the analysis of human hepatocarcinoma cells (Hep G2) for reduced and...	glutathione, HepG2,	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
121	Published Document	2011	Nanotechnology Characterization Laboratory	NCL	ITA-17	In Vitro Induction of Leukoc yte Procoagulant Activity by Nanoparticles	http://ncl.cancer.gov/NCL_Method_ITA-17.pdf	Leukocyte procoagulant activity (PCA) is accepted as an important component in the onset of...	PCA, DIC, APL	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
120	Published Document	2011	Nanotechnology Characterization Laboratory	NCL	ITA-14	Analysis of Nanoparticle Effect s on Maturation of Monocyte Derived Dendritic Cells In Vitro	http://ncl.cancer.gov/NCL_Method_ITA-14.pdf	Dendritic cells (DC) are antigen-presenting cells. They play an important role in initiation and...	dendritic cells, cytotoxicity	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
119	Published Document	2009	Nanotechnology Characterization Laboratory	NCL	ITA-11	Measurement of Nanoparticle Effect s on Cytotoxic Activity of NK Cells by Label-Free RT-CES System	http://ncl.cancer.gov/NCL_Method_ITA-11.pdf	NK cells carry small granules in their cytoplasm which contain special proteins such as perforin...	perforin, NK, granzymes	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Energy e.g. Energy storage, energy efficiency	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
118	Published Document	2011	Nanotechnology Characterization Laboratory	NCL	ITA-9	Phagocytosis Assay	http://ncl.cancer.gov/NCL_Method_ITA-9.pdf	This document describes a protocol for evaluation of nanoparticle internalization by phagocytic...	phagolysosome	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
117	Published Document	2011	Nanotechnology Characterization Laboratory	NCL	ITA-8	Chemotaxis Assay	http://ncl.cancer.gov/NCL_Method_ITA-8.pdf	This document provides a protocol for rapid, quantitative measure of the chemoattractant capacity...	chemokines, chemoattractant capacity, prostaglandins, cytokines	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
116	Published Document	2011	National Institute of Standards and Technology-Nanotechnology Characterization Laboratory	NCL	ITA-7	Detection of Nitric Oxide Producti on by the Macrophage Cell Line RAW264.7	http://ncl.cancer.gov/NCL_Method_ITA-7.pdf	This document describes a protocol for quantitative determination of nitrite (NO2-) a stable...	cytotoxicity, nitricoxide, NO 2, NO 3	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
115	Published Document	2011	Nanotechnology Characterization Laboratory	NCL	ITA-6	Leukocyte Proliferation Assay	http://ncl.cancer.gov/NCL_Method_ITA-6.pdf	This document describes a protocol for assessing the effect of a nanoparticle formulation on the...	PHA-M,lymphocytes	e. Specification	Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
114	Published Document	2009	Nanotechnology Characterization Laboratory	NCL	ITA-3	Mouse Granulocyte-Macrophage Colony-Forming Unit Assay	http://ncl.cancer.gov/NCL_Method_ITA-3.pdf	Myelosuppression is a common dose limiting toxicity of cytotoxic oncology drugs. Nanoparticles...	BM, granulocyte macrophagecolony, Myelosuppression	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
113	Published Document	2011	Nanotechnology Characterization Laboratory	NCL	ITA-12	Coagulation Assays	http://ncl.cancer.gov/NCL_Method_ITA-12.pdf	This document describes a protocol for assessing the effect a nanoparticle formulation may have...	APTT, TT, Coagulation	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
112	Published Document	2010	Nanotechnology Characterization Laboratory	NCL	ITA-5.2	Quantitative Analysis of Complement Activation	http://ncl.cancer.gov/NCL_Method_ITA-5.2.pdf	This document describes a protocol for quantitative determination of complement activation by an...	EIA	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
111	Published Document	2010	Nanotechnology Characterization Laboratory	NCL	ITA-5.1	Qualitative Analysis of Tota l Complement Activation by Western Blot	http://ncl.cancer.gov/NCL_Method_ITA-5.1.pdf	This document describes a protocol for qualitative determination of total complement activation...	Western blot, lectin	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-16
110	Published Document	2010	Nanotechnology Characterization Laboratory	NCL	ITA-4	NCL Method ITA-4 Analysis of Nanoparticle Interaction with Plasma Proteins by 2D PAGE	http://ncl.cancer.gov/NCL_Method_ITA-4.pdf	Proteins bind the surfaces of nanoparticles, and biological materials in general, immediately...	nanoparticles, protein binding, phagocytic cells	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-10
109	Published Document	2009	Nanotechnology Characterization Laboratory	NCL	ITA-2	Analysis of Platelet Aggregation	http://ncl.cancer.gov/NCL_Method_ITA-2.pdf	This document describes a procedure for analysis of platelet aggregation (1-6). Platelets are...	thrombocyte, megakaryocyte, Platelets	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-10
108	Published Document	2009	Nanotechnology Characterization Laboratory	NCL	ITA-1	Analysis of Hemolytic Properties of Nanoparticles	http://ncl.cancer.gov/NCL_Method_ITA-1.pdf	The hemolytic potential of all intravenously administered pharmaceuticals must be evaluated....	in vivo, hemolytic potential, nanoparticles				Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-10
107	Published Document	2009	Nanotechnology Characterization Laboratory	NCL	ITA-1	Analysis of Hemolytic Properties of Nanoparticles	http://ncl.cancer.gov/NCL_Method_ITA-1.pdf	The hemolytic potential of all intravenously administered pharmaceuticals must be evaluated....	in vivo, hemolytic potential, nanoparticles				Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-10
106	Published Document	2013	Nanotechnology Characterization Laboratory	NCL	GTA-13	This protocol assumes an intermediate level of scientific competency with regard to techniques, instrumentation, and safety procedures. Rudimentary assay details have been omitted for the sake of brevity. NCL Method GTA - 13	http://ncl.cancer.gov/NCL_Method_GTA-13.pdf	Characterization of drug release under physiological conditions is very important for prediction...	hydrophilic nanoparticle platform, RBC, in vivo	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-10
105	Published Document	2010	Nanotechnology Characterization Laboratory	NCL	Cell Binding	Cell Binding/Internalization	http://ncl.cancer.gov/NCL_Method_Cell_Binding.pdf	NCL has methods to asses targe ting by measuring the inhibition coefficient (Ki) and binding...	Cell Binding	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-10
104	Published Document	2011	Nanotechnology Characterization Laboratory	NCL	STE-3	Detection of Mycoplasma	http://ncl.cancer.gov/NCL_Method_STE-3.pdf	Nanoparticles submitted to the NCL may be subjected to testing for mycoplasma when deemed...	hybridoma, mycoplasma	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	Admin	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-10
103	Published Document	2011	Nanotechnology Characterization Laboratory	NCL	STE-2	Detection of Microbial Contamination	http://ncl.cancer.gov/NCL_Method_STE-2.pdf	The intended purpose of this assay is to avoid introduction of microbial contamination into in...	in vitro, in vivo, nanomaterial	e. Specification	Measurement and Characterizations Health, Safety, and Environment	Chemicals e.g. pesticides, pharmaceuticals, paints, basic chemicals	A	[email protected]	ANSI	http://ncl.cancer.gov/working_assay-cascade_dow...	EF	sculley	Editors	2014-07-10